Name: TECHNIQUES IN CELLULAR AND MOLECULAR BIOLOGY
Code: 203101001
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: First term
Speciality:
Language: English
Mode of study: On-site class
Lecturer data: WEISS , JULIA ROSL
Knowledge area: Genética
Department: Ingeniería Agronómica
Telephone: 968325777
Email: julia.weiss@upct.es
Office hours and location:
miércoles - 13:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.15
Se ruega contactar previamente por correo electrónico indicando emotivo de la tutoría.
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 4
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location: Contact with the lecturer
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 5
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data:
Lecturer data: PALOP GÓMEZ, ALFREDO
Knowledge area: Tecnología de Alimentos
Department: Ingeniería Agronómica
Telephone: 968325762
Email: alfredo.palop@upct.es
Office hours and location:
lunes - 10:00 / 10:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.25
Para cualquier tutoría, contactar previamente por correo electrónico
Qualifications/Degrees:
PhD in Veterinary from University of Zaragoza (SPAIN) - 1995
Academic rank in UPCT: Catedrático de Universidad
Number of five-year periods: 5
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
[CB6 ]. To possess and understand knowledge that provides a basis or opportunity to be original in the development and / or application of ideas, often in a research context.
[CG1 ]. To learn about the field of study in which agricultural and food research and development are developed and the research skills and methods related to such field.
[CG4 ]. To be able to perform a critical analysis, and an evaluation and synthesis analysis of new and complex ideas in the agri-food sector.
[CE1 ]. To synthesize previous knowledge together with newly acquired knowledge in the matter, so as to be able to cope in agri-food contexts in which there is little specific information.
[CE3 ]. To show a certain degree of scientific and technical training to start a research activity in the field of agri-food.
[CE4 ]. To show a certain degree of understanding of the concepts, principles and theories related to the chosen subjects of the master's program.
Competencias específicas de la asignatura: 1.- Identificar diferentes técnicas de análisis de ADN - secuenciación y análisis de punto de fusión. 2.- Conocer la técnica de microscopía electrónica de barrido. 3.- Conocer la técnica de cromatografía de gases-espectrometría de masas. 4.- Conocer el funcionamiento de la técnica de HPLC. 5.- Conocer la técnica de citometría de flujo. 6.- Conocer la técnica de electroforésis de proteínas. Aspectos referidos a la semipresencialidad: Los estudiantes pueden participar en las actividades formativas de esta asignatura de forma presencial (acudiendo físicamente al espacio en el que se desarrollan) o en línea a través de internet, excepto en "Sesiones Prácticas de laboratorio, campo o planta piloto" en las que solo puede participarse de forma presencial.
[CT1 ]. Spoken and written effective communication
[CT4 ]. Using information resources responsibly
1.1.- Describir los conceptos básicos sobre la secuenciación de ADN en el ámbito de
secuenciación Sanger y Next-Generation Sequencing.
1.2.- Explicar los conceptos básicos de la microscopía electrónica de barrido.
1.3.- Identificar los principios básicos de la separación de metabolitos por HPLC.
1.4.- Listar el funcionamiento y utilidad de la citometría de flujo en el ámbito agroalimentario.
1.5.- Identificar los principios básicos de la proteómica y separación de proteínas.
1.6.- Explicar el funcionamineto de GC-MS y sus aplicaciones en la industria agroalimentaria.
1.- Secuenciación de ADN Sanger y Next-Generation Sequencing. 2.- Microscopía electrónica de barrido. 3.- HPLC. 4.- Citometría de flujo. 5.- Proteómica y separación de proteínas. 6.- GC-MS.
Teaching unit 1 - Analysis of metabolites by HPLC
1.1.Preparation of samples for HPLC analysis
1.2.Components and methods in HPLC operations
1.3.Application of HPLC technique
1.4.Guide for problems that can occur during HPLC operation
Teaching unit 2 - Analysis of metabolites by gas chromatography
2.1.Introduction to chromatography
Definition
Basic principles
Components of a gas chromatograph, hazards and critical control points.
Identification of compounds, quantification
Recent innovations
2.2.Application in the agro-alimentary field (composition, no exogenous, foreign elements)
Simple gases and headspace with and without micro extraction in solid phase and/or derivatisation.
Other applications: Purge and trap, electronic nose, non-destructive systems, stir bar sorptive extraction, headspace aroma fingerprinting, static and dynamic (continuous) systems.
Examples in the agro-alimentary field and extraction and analysis of volatile aromas from plant tissues.
Teaching unit 3 - Analysis of proteins by mono ¿ and bi-dimensional electrophoresis
3.1. Introduction. Basic principles of electrophoresis
3.2. Electrophoretic techniques
Polyacrylamide gel electrophoresis: native, SDS and isoelectric focusing.
Bi-dimensional electrophoresis. Preparation of samples. First dimension. Second dimension. Protein detection. Proteomic analysis.
Teaching unit 4 - Flow cytometry
4.1 Fundamentals of the technique
4.2 Optic and electronic of a flow cytometer
4.3 Cell sorting
4.4 Applications and practical examples.
Teaching unit 5 - The scanning electron microscope
5.1 Main concepts of the technique
5.2 Structure and functioning
5.2 Sample preparation
5.3 Applications, advantages and disadvantages
Teaching unit 6 - DNA sequence analysis
6.1 Introduction to DNA sequence analysis
6.2 Basic molecular techniques with relevance for sequencing and melting peak analysis.
6.3 Sequencing methods ¿ Sanger; pyrosequencing; next generation sequencing.
6.4 DNA sequence analysis by melting peak analysis and high resolution melting analysis.
6.5 Applications
Teaching unit 1
- Extraction and analysis of metabolites by HPLC
Teaching unit 2
- Extraction and analysis of metabolites by gas chromatography
Teaching unit 3
- Analysis of proteins by mono- and bi-dimensional electrophoresis
Teaching unit 4
- Basic handling of the flow cytometer
Teaching unit 5
- Basic handling of the scanning electron microscope
Teaching unit 6
- Basic handling and applications of a next generation sequencing device and a thermocycler for real-time PCR
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Theory class: Activities consisting of training sessions to develop theoretical knowledge based on concepts and theories
Lectures given by the professors responsible for the six teaching modules
26
100
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving exercises, or practical cases
0
100
Laboratory or field practice class: Activities aimed at developing practical or applied skills by the student supervised by a remote teacher
Practical class in the laboratories (the practicals correspond to the different teaching modules)
10
100
Practical class in the computer room: Activities for the acquisition of certain skills through the use of specific software
0
100
Seminars, tutorials led by teaching staff, conferences, visits, round tables, etc .: Activities to develop theoretical, practical or applied knowledge based on specific topics or views of the profession
0
100
Assessments (continuous assessment system): speaking or writing tests, presentations, individually or in groups, which should include the knowledge acquired in the classes. Formative and summative assessment activities are included here
Completion of a written exam, presentation of a scientific article
4
100
Assessments (final assessment system): speaking or writing tests, individually or in groups, which should include the knowledge acquired in the classes. Summative evaluation is included
Completion of final exams (written exam, presentation of a scientific article)
4
100
Tutorials: Individual or in groups, will serve to advise, resolve any doubts, guide, monitor work or the knowledge acquired
Tutorials (presentially or online) with the professors responsible for the teaching modules
4
50
Carrying out individual or group assignments: Autonomous and / or collaborative learning to develop theoretical, practical or applied knowledge by carrying out projects, practice reports and / or assignments
Preparation of individual oral presentations
20
0
Individual study: Time dedicated to studying the subject
Individual study of the subjects treated in the classes of theory and in practicals
52
0
Individual official test
Written exam; 2 questions / module (6 modules) oriented fundamentally to evaluate the learning of basic concepts.
65 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Presentation, during a maximum of 15 minutes, with 5 minutes of questions, of an assignment. In this work, the students will synthesize their knowledge about a subject of mutual agreement with the teacher.
35 %
Individual official test
Written exam; 2 questions / module (6 modules) oriented fundamentally to evaluate the learning of basic concepts.
65 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Presentation, during a maximum of 15 minutes, with 5 minutes of questions, of an assignment. In this work, the students will synthesize their knowledge about a subject of mutual agreement with the teacher.
35 %
Performance of small tests of short duration in class
Author: Glick, Bernard R.
Title: Molecular biotechnology: principles and applications of recombinant DNA
Editorial: ASM press
Publication Date: 1998
ISBN: 1555811361
Author: Dashek, William V.
Title: Methods in plant biochemistry and molecular biology
Editorial: CRC
Publication Date: 1997
ISBN: 9781315895383
Author: Núñez, Rafael
Title: Flow cytometry for research scientists principles and applications
Editorial: Horizon
Publication Date: 2001
ISBN: 1898486263
Author:
Title: Genome sequencing technology and algorithms
Editorial: Artech House,
Publication Date: 2008
ISBN: 9781596930940
Author: Aballe Caride, Miguel
Title: Microscopía electrónica de barrido y microanálisis por rayos X
Editorial: Consejo Superior de Investigaciones Científicas, : Rueda
Publication Date: 1996
ISBN: 8472070944
Author: Brown, T.A.
Title: Genomes 3
Editorial: Wiley-Liss
Publication Date: 2007
ISBN: 0815341385
Author: Cserháti, Tibor
Title: Chromatography in food science and technology
Editorial: Technomic
Publication Date: 1999
ISBN: 1566767490
Author: ,
Title: I Reunión Científica en Biología Celular y Molecular análisis y metodologías en diagnóstico y terapia génica: [La Coruña, noviembre 1992]
Editorial: Universidade, Servicio de Publicacions
Publication Date: 1993
ISBN: 8488301693
Author: Grimstone, Albert Victor
Title: El microscopio electrónico en Biología /
Editorial: Omega,
Publication Date: 1981
ISBN:
Author: Westermeier, Reiner
Title: Proteomics in practice a laboratory manual of photeome analysis
Editorial: Wiley-VCH
Publication Date: 2008
ISBN: 9783527319411
Author: Skoog, Douglas A.
Title: Principios de análisis instrumental
Editorial: McGraw-Hill
Publication Date: 2018
ISBN: 9786075266558
Author: Izquierdo Rojo, Marta
Title: Ingeniería genética y transferencia génica
Editorial: Pirámide
Publication Date: 2001
ISBN: 8436815637
Author:
Title: A-Z of quantitative PCR
Editorial: International University Line,
Publication Date: 2009
ISBN: 9780963681782
Author: Mullis
Title: Reacción en cadena de la polimerasa.
Editorial: Investigación y Ciencia 165: 30-47.
Publication Date: 1990
ISBN:
Author: Ballester, P.
Title: Cromatografía de gases
Editorial: University of Richmond. UIB.
Publication Date: 2003
ISBN:
Author: Banks, N.H. et al..
Title: Proposal for a rationalized system of units for postharvest research in gas exchange.
Editorial: HortScience 30: 1129-1131.
Publication Date: 1995
ISBN:
Author: Morozova and Marra
Title: Applications of next-generation sequencing Technologies in functional genomics.
Editorial: Genomics 92: 255-264.
Publication Date: 2008
ISBN:
Author: Kader, A.A.
Title: Methods of gas mixing, sampling and analysis
Editorial: En: Kader, A.A. (ed.) Postharvest Technology of Horticultural Crops. Publication No. 3311.Division of Agriculture and Natural Resources, University of California, CA. pp. 93-96.
Publication Date: 1999
ISBN:
Author: Saltveit, M.E.
Title: Measuring respiration.
Editorial: http://www.ucdavis.edu 5 pp.
Publication Date: 2003
ISBN:
Teaching unit 1:
High performance liquid chromatography: A users guide
http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html
http://ull.chemistry.uakron.edu/chemsep/gc/
http://www.shsu.edu/~chm_tgc/sounds/sound.htmlhttp://www.netaccess.on.ca/~dbc/cic_hamilton/liquid.html
Teaching unit 2:
Chromatography network. http://www.chromatographynetwork.com
http://www.uib.es/depart/dqu/dquo/pau/Cromatograf%92a/chrom10/chrom/GC/concept/main.htm
Chromatography hazards.
http://129.93.84.115/Chemistry/DoChem/DoChem128.html#Hazards
Teaching unit 6:
http://www.ornl.gov/sci/techresources/Human_Genome/faq/seqfacts.shtml
http://biotech.about.com/od/pcr/a/sequencing.htm
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.dnalc.org/resources/animations/pcr.html
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.gene-quantification.de/hrm.html
http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html
Virtual classroom