Name: ADVANCED TECHNIQUES IN VITRO PLANT CULTURE: MICROPROPAGATION AND PRODUCTION OF VALUABLE COMPOUNDS
Code: 203301003
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 1st Year - First term
Speciality:
Language: English
Mode of study: On-site class
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
[CB6 ]. To possess and understand knowledge that provides a basis or opportunity to be original in the development and / or application of ideas, often in a research context.
[CG1 ]. To learn about the field of study in which agricultural and food research and development are developed and the research skills and methods related to such field.
[CG4 ]. To be able to perform a critical analysis, and an evaluation and synthesis analysis of new and complex ideas in the agri-food sector.
[CE1 ]. To synthesize previous knowledge together with newly acquired knowledge in the matter, so as to be able to cope in agri-food contexts in which there is little specific information.
[CE3 ]. To show a certain degree of scientific and technical training to start a research activity in the field of agri-food.
[CE4 ]. To show a certain degree of understanding of the concepts, principles and theories related to the chosen subjects of the master's program.
Competencias específicas de la asignatura:
1. Interpretar, discutir críticamente y transmitir oralmente artículos de investigación sobre el tema.
2. Detectar errores de diseño y buscar soluciones a los mismos en instalaciones dedicadas al cultivo in vitro de material vegetal.
3. Dominar las técnicas de manipulación empleadas en el cultivo in vitro de material vegetal con el fin de multiplicarlo, conservarlo y obtener metabolitos de interés industrial.
Aspectos referidos a la semipresencialidad:
Los estudiantes pueden participar en las actividades formativas de esta asignatura de forma presencial (acudiendo físicamente al espacio en el que se desarrollan) o en línea a través de internet, excepto en "Sesiones Prácticas de laboratorio, campo o planta piloto", en las que solo puede participarse de forma presencial.
[CT1 ]. Spoken and written effective communication
[CT4 ]. Using information resources responsibly
Conocer los factores que determinan las respuestas del material vegetal cultivado in vitro y la forma de modificarlos.
Analizar, explicar y discutir las características de instalaciones y procesos dedicados al cultivo in vitro.
Proponer procesos nuevos, o mejoras de los existentes, para optimizar y rentabilizar la producción de plantas y/o la producción de metabolitos por material cultivado in vitro.
Revisión de metodologías existentes y análisis de las tendencias actuales en la aplicación de técnicas de cultivo in vitro de material vegetal para su propagación, conservación y obtención de productos con alto valor añadido.
Unit 1: Introduction and requeriments
C1. Introduction
C2. Facilities and equipment in a micropropagation laboratory
C3. Aseptic work conditions
Unit 2: Micropropagation: methods, limitacions and aplications
C4. Micropropagation steps
C5. Multiplication methods
C6. Automation and large-scale cultures
C7. Plant micropropagation limitations
C8. Common problems associated with micropropagation
C9. Applications
Unit 3: Biosynthetic potential of plants
C10. Introduction to the study of the plant¿s biosynthetic potential
C11. In vitro culture of plant cells, tissues and organs
C12. Special productions techniques: addition of precursors, elicitation, biotransformation, mixed cultures
C13. Industrial scale cultures
C14. Applications
Unit 1-3
Practice 1 (P1). Construction of a bioreactor for the micropropagation of plant material and monitoring of crops (4 h). Practice 2 (P2). Elicitation of cell cultures and analysis of the production of bioactive compounds (4 h). Practice 3 (P3). Establishment of root crops for the production of secondary metabolites (2 h).
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Presentation of the lecture. Resolve queries from students. To develop theoretical knowledge based on concepts and theories
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving
exercises, or practical cases
26
100
Class in laboratory: practical classes / internships
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
10
100
Assessment activities (continuous assessment system)
Continuous assessment system
4
50
Assessment activities (final assessment system)
Attendance to the exams
4
50
Tutorials
Making suggestions for improvement of student work. Resolve doubts.
3
0
Student work: study or individual or group work
Preparation oral exposition
Preparation a formal lab report.
Preparation oral exposition.
Literature search
Self-study: Study the lectures
73
0
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions
30 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
20 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
45 %
Other evaluation activities
Students will be asked to make a self-assessment report of their oral presentation, as well as an evaluation of the oral exposure of all their classmates.
5 %
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions
35 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
20 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
45 %
Author: Altman, A.
Title: Agricultural biotechnology
Editorial: Marcel Dekker
Publication Date: 1998
ISBN: 0824794397
Author: Davey, Michael R.
Title: Plant cell culture essential methods
Editorial: Wiley-Blackwell,
Publication Date: 2010
ISBN: 9780470686485
Author:
Title: Plant tissue culture engineering
Editorial: Springer
Publication Date: 2006
ISBN: 9781402035944
Aula virtual (several research articles related with the different topics of this subject will be made available on the aula virtual)
http://plant-tc.cfans.umn.edu/listserv/
Name: ADVANCED TECHNIQUES IN VITRO PLANT CULTURE: MICROPROPAGATION AND PRODUCTION OF VALUABLE COMPOUNDS
Code: 203301003
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 1st Year - First term
Speciality:
Language: English
Mode of study: Semi on-site class
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
[CB6 ]. To possess and understand knowledge that provides a basis or opportunity to be original in the development and / or application of ideas, often in a research context.
[CG1 ]. To learn about the field of study in which agricultural and food research and development are developed and the research skills and methods related to such field.
[CG4 ]. To be able to perform a critical analysis, and an evaluation and synthesis analysis of new and complex ideas in the agri-food sector.
[CE1 ]. To synthesize previous knowledge together with newly acquired knowledge in the matter, so as to be able to cope in agri-food contexts in which there is little specific information.
[CE3 ]. To show a certain degree of scientific and technical training to start a research activity in the field of agri-food.
[CE4 ]. To show a certain degree of understanding of the concepts, principles and theories related to the chosen subjects of the master's program.
Competencias específicas de la asignatura:
1. Interpretar, discutir críticamente y transmitir oralmente artículos de investigación sobre el tema.
2. Detectar errores de diseño y buscar soluciones a los mismos en instalaciones dedicadas al cultivo in vitro de material vegetal.
3. Dominar las técnicas de manipulación empleadas en el cultivo in vitro de material vegetal con el fin de multiplicarlo, conservarlo y obtener metabolitos de interés industrial.
Aspectos referidos a la semipresencialidad:
Los estudiantes pueden participar en las actividades formativas de esta asignatura de forma presencial (acudiendo físicamente al espacio en el que se desarrollan) o en línea a través de internet, excepto en "Sesiones Prácticas de laboratorio, campo o planta piloto", en las que solo puede participarse de forma presencial.
[CT1 ]. Spoken and written effective communication
[CT4 ]. Using information resources responsibly
Conocer los factores que determinan las respuestas del material vegetal cultivado in vitro y la forma de modificarlos.
Analizar, explicar y discutir las características de instalaciones y procesos dedicados al cultivo in vitro.
Proponer procesos nuevos, o mejoras de los existentes, para optimizar y rentabilizar la producción de plantas y/o la producción de metabolitos por material cultivado in vitro.
Revisión de metodologías existentes y análisis de las tendencias actuales en la aplicación de técnicas de cultivo in vitro de material vegetal para su propagación, conservación y obtención de productos con alto valor añadido.
Unit 1: Introduction and requeriments
C1. Introduction
C2. Facilities and equipment in a micropropagation laboratory
C3. Aseptic work conditions
Unit 2: Micropropagation: methods, limitacions and aplications
C4. Micropropagation steps
C5. Multiplication methods
C6. Automation and large-scale cultures
C7. Plant micropropagation limitations
C8. Common problems associated with micropropagation
C9. Applications
Unit 3: Biosynthetic potential of plants
C10. Introduction to the study of the plant¿s biosynthetic potential
C11. In vitro culture of plant cells, tissues and organs
C12. Special productions techniques: addition of precursors, elicitation, biotransformation, mixed cultures
C13. Industrial scale cultures
C14. Applications
Unit 1-3
Practice 1 (P1). Construction of a bioreactor for the micropropagation of plant material and monitoring of crops (4 h). Practice 2 (P2). Elicitation of cell cultures and analysis of the production of bioactive compounds (4 h). Practice 3 (P3). Establishment of root crops for the production of secondary metabolites (2 h).
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Presentation of the lecture. Resolve queries from students. To develop theoretical knowledge based on concepts and theories
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving
exercises, or practical cases
26
0
Class in laboratory: practical classes / internships
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
10
100
Assessment activities (continuous assessment system)
Continuous assessment system
4
0
Assessment activities (final assessment system)
Attendance to the exams
4
0
Tutorials
Making suggestions for improvement of student work. Resolve doubts.
3
0
Student work: study or individual or group work
Preparation oral exposition
Preparation a formal lab report.
Preparation oral exposition.
Literature search
Self-study: Study the lectures
73
0
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions
30 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
20 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
45 %
Other evaluation activities
Students will be asked to make a self-assessment report of their oral presentation, as well as an evaluation of the oral exposure of all their classmates.
5 %
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions
35 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
20 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
45 %
Author: Altman, A.
Title: Agricultural biotechnology
Editorial: Marcel Dekker
Publication Date: 1998
ISBN: 0824794397
Author: Davey, Michael R.
Title: Plant cell culture essential methods
Editorial: Wiley-Blackwell,
Publication Date: 2010
ISBN: 9780470686485
Author:
Title: Plant tissue culture engineering
Editorial: Springer
Publication Date: 2006
ISBN: 9781402035944
Aula virtual (several research articles related with the different topics of this subject will be made available on the aula virtual)
http://plant-tc.cfans.umn.edu/listserv/