Name: PLANT DEFENSE. BIOTECHNOLOGICAL TOOLS TO OBTAIN RESISTANT PLANTS TO DISEASES
Code: 229102011
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 2nd Year - First term
Speciality:
Language: English
Mode of study: On-site class
Lecturer data: EGEA GILABERT, CATALINA
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325520 - 968325706
Email: catalina.egea@upct.es
Office hours and location:
lunes - 09:00 / 11:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.18
Se ruega contactar previamente con la profesora por correo electrónico
martes - 16:00 / 18:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.18
Se ruega contactar previamente con la profesora mediante correo electrónico
miércoles - 09:00 / 11:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.18
Se ruega contactar previamente con la profesora vía correo electrónico
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación
Curriculum Vitae: Full Profile
Se trata de una asignatura optativa de la titulación que puede cursarse si se elige el módulo optativo de formación investigadora. Las competencias de dicho Módulo a adquirir son:
Técnicas Avanzadas de cultivo in vitro:
- Capacidad para interpretar, discutir críticamente y transmitir oralmente artículos de investigación sobre el tema.
- Capacidad para detectar errores de diseño y para buscar soluciones dedicadas al cultivo in vitro.
- Dominio de las técnicas de manipulación empleadas en el cultivo in vitro de material vegetal.
- Capacidad para poner en marcha y optimizar procesos de obtención de compuestos químicos con interés industrial mediante cultivo in vitro de material vegetal.
- Capacidad para discernir sobre la rentabilidad de un proceso de producción de plantas y/o metabolitos mediante el uso de técnicas de cultivo in vitro.
La defensa vegetal:
- Capacidad para interpretar, discutir críticamente y transmitir oralmente artículos de investigación sobre el tema.
- Capacidad para encontrar soluciones para el control de enfermedades en cultivos.
Es recomendable que el alumno tenga buenos fundamentos en Biología, Fisiología Vegetal y Genética.
Técnicas Avanzadas de cultivo in vitro: micropropagación y producción de compuestos de interés industrial.
- Conocer los factores que determinan las respuestas del material vegetal cultivado in vitro y la forma de modificarlos.
- Analizar, explicar y discutir las características de instalaciones y procesos dedicados al cultivo in vitro.
- Proponer procesos nuevos, o mejoras de los existentes, para optimizar y rentabilizar la producción de plantas y/o la producción de metabolitos por material cultivado in vitro.
La defensa vegetal: Herramientas biotecnológicas para la obtención de plantas resistentes a las enfermedades.
- Conocer los mecanismos generales de acción de los diferentes patógenos vegetales.
- Distinguir entre defensa vegetal activa y pasiva.
- Explicar en qué consisten los mecanismos de defensa pasiva en plantas.
- Explicar en qué consisten los mecanismos de defensa activa en plantas.
- Conocer las bases genéticas de la interacción planta-patógeno.
- Distinguir entre los diferentes tipos de resistencias sistémicas.
- Conocer las herramientas biotecnológicas existentes para la inducción de resistencia en plantas.
Técnicas avanzadas de cultivo in vitro: micropropagación y producción de compuestos de interés industrial. 1. Introducción. 2. Limitaciones de la micropropagación vegetal. 3. Aplicaciones de las técnicas de micropropagación. 4. Impacto económico de la micropropagación. Principales productores y mercado de las plantas micropropagadas. 5. Introducción al estudio del potencial biosintético de las plantas. Aprovechamiento industrial. 6. Cultivos a escala industrial. 7. Aplicaciones de las técnicas de producción in vitro de metabolitos. La defensa vegetal. Herramientas biotecnológicas para la obtención de plantas resistentes a las enfermedades. 1. Introducción. 2. Patógenos. 3. Sistemas de defensa vegetal. Defensa pasiva. 4. Sistemas de defensa vegetal. Defensa activa. 5. Bases genética y molecular de la interacción planta-patógeno 6. Resistencias sistémicas. 7. Herramientas Biotecnológicas y uso de recursos genéticos.
Unit 1. INTRODUCTION
1.1. INTRODUCTION. Plant anatomy. Plant physiology.
1.2. DEFINITIONS AND CONCEPTS. Pathogen, host, elicitor, resistance, susceptibility, immunity, virulence and avirulence. Plant¿pathogen interaction, compatible and incompatible.
Unit 2. PATHOGENS
2.1. Types of pathogens: viruses, bacteria, fungi, nematodes, insects, parasitic plants. Mechanism of invasion.
Unit 3. PLANT DEFENCE SYSTEMS
3.1. Passive defence.
3.2. Active defence.
Unit 4. GENETICS AND MOLECULAR BASES OF PLANT¿PATHOGEN INTERACTION
4.1. Monogenicoligogenic/polygenic resistance. General resistance and specific resistance. Vertical and horizontal resistance. Resistance genes.
Unit 5. SYSTEMIC RESISTANCE. SIGNALLING OF THE DEFENCE.
5.1. Systemic acquired resistance.
5.2. Induced systemic resistance.
Unit 6. INDUCTION OF RESISTANCE IN PLANTS
6.1. Biotechnological tools to induce resistance in plants.
6.2. Microbial induction. Topical application of inducers.
6.3. Integration of induced resistance in crop production.
There is no laboratory practices in this course
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Theory class: Activities consisting of training sessions to develop theoretical knowledge based on concepts and theories
Master class interspersed with cooperative learning techniques casual. Resolution of doubts raised by the students
38
100
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving exercises, or practical cases
0
100
Laboratory or field practice class: Activities aimed at developing practical or applied skills by the student supervised by a remote teacher
Oral presentation of a research article.
0
100
Practical class in the computer room: Activities for the acquisition of certain skills through the use of specific software
0
100
Seminars, tutorials led by teaching staff, conferences, visits, round tables, etc .: Activities to develop theoretical, practical or applied knowledge based on specific topics or views of the profession
Oral presentation of a research article that has previously been worked on it.
2
100
Assessment activities (final assessment system)
Official written examination.
4
100
Tutorials: Individual or in groups, will serve to advise, resolve any doubts, guide, monitor work or the knowledge acquired
Resolution of doubts and additional explanations both face to face and remote way.
6
50
Carrying out individual or group assignments: Autonomous and / or collaborative learning to develop theoretical, practical or applied knowledge by carrying out projects, practice reports and / or assignments
Discuss and analyze a research article. Search for information. Preparation of the report, abstract or conceptual map. Preparation of the oral presentation. Study work.
70
0
Individual official test
Written exam with 20 multiple choice questions (50%) and 2 short answer questions (50%).
50 %
Presentation and defence of individual or group assignments
Elaboration of individual or group works on a research article and oral presentation of it to the classmates. Students who are unable to performance the oral presentation, for a justified reason, must carry out additional a work proposed by the teacher
45 %
Other evaluation activities
Attendance and participation in class. Students, who are not able to attend the class, must carry out a bibliographic search on a topic proposed by the teacher
5 %
Individual official test
Written exam with 20 multiple choice questions (50%) and 2 short answer questions (50%).
50 %
Presentation and defence of individual or group assignments
Elaboration of individual or group works on a research article and oral presentation of it to the classmates. Students who are unable to performance the oral presentation, for a justified reason, must carry out additional a work proposed by the teacher
45 %
Other evaluation activities
Attendance and participation in class. Students, who are not able to attend the class, must carry out a bibliographic search on a topic proposed by the teacher
5 %
Students who cannot attend class should be contact to the teacher at the begining of the course.
Author: Agrios, George N.
Title: Plant pathology
Editorial: Elsevier Academic Press,
Publication Date: 2005
ISBN: 9780120445653
Author: Taiz, Lincoln
Title: Plant physiology and development
Editorial: Sinauer,
Publication Date: 2015
ISBN: 9781605352558
Author: Buchanan, Bob B.
Title: Biochemistry and molecular biology of plants
Editorial: Wiley John
Publication Date: 2015
ISBN: 0470714212
Amen et al. (2010). Methyl salicylate production in tomato affects biotic interactions. Plant Journal 62: 124¿134.
Baldwin et al. (2002). Volatile signalling in plant¿plant¿herbivore interactions: what is real? Current Opinion in Plant Biology 5: 351¿354.
Barrios Perez & Brown (2014). The role of ROS signaling in cross¿tolerance: from model to crop. Frontiers in Plant Science 5: 754.
Berger (2002). Jasmonate¿related mutants of Arabidopsis as tools for studying stress signalling. Planta 214: 497¿504.
Conrath et al. (2006). Priming: Getting Ready for Battle. Molecular Plant¿Microbe Interactions 10: 1062¿1071.
Dangl & Jones (2001). Plant pathogens and integrated defence responses to infection. Nature 411: 826¿833.
Dickinson & Beynon (2000). Molecular Plant Pathology. Dheffield Academic Press Ltd. (UK). Feys & Parker. (2000). Interplay of signalling pathways in plant disease resistance. Trends in Genetics 16: 449¿455.
Horbach et al. (2011). When and how to kill a plant cell: Infection strategies of plant pathogenic fungi. Journal of Plant Physiology 168: 51¿62.
Kunkel & Brooks (2002) Cross talk between signalling pathways in pathogen defense. Current Opinion in Plant Biology 5: 325¿331.
Lorenzo & Solano (2005). Molecular players regulating the jasmonate signalling network. Current Opinion in Plant Biology 8: 532¿540.
Luna et al. (2012). Next¿Generation Systemic Acquired Resistance. Plant Physiology 158: 844¿853.
Malik (2011). GSNOR¿mediated de¿nitrosylation in the plant defence response. Plant Science 181: 540¿ 544.
Mauch¿Mani & Mauch (2005). The role of abscisic acid in plant¿pathogen interactions. Current Opinion in Plant Biology 8: 409¿414.
Prell & Day (2001). Plant fungal pathogen interaction. Ed. Springer¿Verlag, Berlin¿New York. Torres (2010). ROS in biotic interactions. Physiologia Plantarum 138: 414¿429.
Vallad & Goodman (2004). Systemic Acquired Resistance and Induced Systemic Resistance in Conventional Agriculture. Crop Science 44: 1920¿1934.
Van Loon et al. (2006). Ethylene as a modulator of disease resistance in plants. Trends in Plant Science 11: 184¿191.
Vlot et al. (2009). Salicylic acid, a multifaceted hormone to combat disease. Annual Review of Phytopathology 47: 177¿206.
Vranova et al. (2002). Signal transduction during oxidative stress. Journal of Experimental Botany 53: 1227¿1236.
Walters et al. (2007). Induced resistance for plant defence. A sustainable approach to crop protection. Blackwell Publishing. Oxford (UK).
Wiermer et al. (2005). Plant immunity: the EDS1 regulatory node. Current Opinion in Plant Biology 8: 383¿389.
Wulff & Moscou (2014). Strategies for transferring resistance into wheat: from wide crosses to GM cassettes. Frontiers in Plant Science 5: 692.
http://www.pathoplant.de./
http://www.genome.jp/kegg/pathway/ath/ath04626.html