Name: TECHNIQUES IN CELLULAR AND MOLECULAR BIOLOGY
Code: 203401001
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 1st Year - First term
Speciality: Especialidad en Tecnología de Alimentos y Biotecnología Agroalimentaria
Language: English
Mode of study: On-site class
Lecturer data: WEISS , JULIA ROSL
Knowledge area: Genética
Department: Ingeniería Agronómica
Telephone: 968325777
Email: julia.weiss@upct.es
Office hours and location:
miércoles - 13:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.15
Se ruega contactar previamente por correo electrónico indicando emotivo de la tutoría.
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 5
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G1, G2
Lecturer data: WEISS , JULIA ROSL
Knowledge area: Genética
Department: Ingeniería Agronómica
Telephone: 968325777
Email: julia.weiss@upct.es
Office hours and location:
miércoles - 13:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.15
Se ruega contactar previamente por correo electrónico indicando emotivo de la tutoría.
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 5
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G1, G2
Lecturer data: PALOP GÓMEZ, ALFREDO
Knowledge area: Tecnología de Alimentos
Department: Ingeniería Agronómica
Telephone: 968325762
Email: alfredo.palop@upct.es
Office hours and location: Tutorials will be carried out by request of the student sending an email to alfredo.palop@upct.es
Qualifications/Degrees:
PhD in Veterinary from University of Zaragoza (SPAIN) - 1995
Academic rank in UPCT: Catedrático de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: PALOP GÓMEZ, ALFREDO
Knowledge area: Tecnología de Alimentos
Department: Ingeniería Agronómica
Telephone: 968325762
Email: alfredo.palop@upct.es
Office hours and location: Tutorials will be carried out by request of the student sending an email to alfredo.palop@upct.es
Qualifications/Degrees:
PhD in Veterinary from University of Zaragoza (SPAIN) - 1995
Academic rank in UPCT: Catedrático de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
1.Identify different DNA analysis techniques, including sequencing and melting point analysis.
2.Understand the technique of scanning electron microscopy (SEM).
3.Understand the technique of gas chromatography¿mass spectrometry (GC¿MS).
4.Understand how high-performance liquid chromatography (HPLC) works.
5.Understand the technique of flow cytometry.
6.Understand the technique of protein electrophoresis.
1.Describe the basic concepts of DNA sequencing, including Sanger sequencing and Next-Generation Sequencing (NGS).
2.Explain the basic principles of scanning electron microscopy (SEM).
3.Identify the basic principles of metabolite separation by high-performance liquid chromatography (HPLC).
4.List the operation and applications of flow cytometry in the agri-food sector.
5.Identify the basic principles of proteomics and protein separation.
6.Explain the operation of gas chromatography¿mass spectrometry (GC¿MS) and its applications in the agri-food industry.
1. Sanger DNA Sequencing and Next-Generation Sequencing (NGS). 2. Scanning Electron Microscopy (SEM). 3. High-Performance Liquid Chromatography (HPLC). 4. Flow Cytometry. 5. Proteomics and Protein Separation. 6. Gas Chromatography¿Mass Spectrometry (GC¿MS).
Teaching unit 1 - Analysis of metabolites by HPLC
1.1.Preparation of samples for HPLC analysis
1.2.Components and methods in HPLC operations
1.3.Application of HPLC technique
1.4.Guide for problems that can occur during HPLC operation
Teaching unit 2 - Analysis of metabolites by gas chromatography
2.1.Introduction to chromatography
Definition
Basic principles
Components of a gas chromatograph, hazards and critical control points.
Identification of compounds, quantification
Recent innovations
2.2.Application in the agro-alimentary field (composition, no exogenous, foreign elements)
Simple gases and headspace with and without micro extraction in solid phase and/or
derivatisation.
Other applications: Purge and trap, electronic nose, non-destructive systems, stir bar sorptive
extraction, headspace aroma fingerprinting, static and dynamic (continuous) systems.
Examples in the agro-alimentary field and extraction and analysis of volatile aromas from plant
tissues.
Teaching unit 3 - Analysis of proteins by mono ¿ and bi-dimensional electrophoresis
3.1. Introduction. Basic principles of electrophoresis
3.2. Electrophoretic techniques
Polyacrylamide gel electrophoresis: native, SDS and isoelectric focusing.
Bi-dimensional electrophoresis. Preparation of samples. First dimension. Second dimension.
Protein detection. Proteomic analysis.
Teaching unit 4 - Flow cytometry
4.1 Fundamentals of the technique
4.2 Optic and electronic of a flow cytometer
4.3 Cell sorting
4.4 Applications and practical examples.
Teaching unit 5 - The scanning electron microscope
5.1 Main concepts of the technique
5.2 Structure and functioning
5.2 Sample preparation
5.3 Applications, advantages and disadvantages
Teaching unit 6 - DNA sequence analysis
6.1 Introduction to DNA sequence analysis
6.2 Basic molecular techniques with relevance for sequencing and melting peak analysis.
6.3 Sequencing methods ¿ Sanger; pyrosequencing; next generation sequencing.
6.4 DNA sequence analysis by melting peak analysis and high resolution melting analysis.
6.5 Applications
Teaching unit 1
- Extraction and analysis of metabolites by HPLC
Teaching unit 2
- Extraction and analysis of metabolites by gas chromatography
Teaching unit 3
- Analysis of proteins by mono- and bi-dimensional electrophoresis
Teaching unit 5
- Basic handling of the scanning electron microscope
Teaching unit 6
- Basic handling and applications of a thermocycler forreal-time PC and high resolution melting analysis
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual") under the "Act in an Emergency" section, "Technical Guides" tab, with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find, in the "Act in an Emergency", sectionrecommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Teaching technique:
Basic program
Presentation of the lecture.
Preparation and provision of
documentation to the virtual
classroom
Resolve queries from students
Students work:
In the class:
Assistance
Personal notes on the documentation
received. Posing of questions and
doubts.
26
100
Class in laboratory: practical classes / internships
Teaching Technique:
Presentation and direction
Students work:
Assistance, development and
presentation
10
100
Continuous assessment activities during class hours.
Theoretical exam in proportion to
each thematic unit of the subject.
Course work (15 minutes / student).
Attendance at the presentation of all
students with discussion and
questions.
4
100
Final and ongoing assessment activities outside class hours.
Theoretical exam in proportion to
each thematic unit of the subject.
Course work (15 minutes / student).
Attendance at the presentation of all
students with discussion and
questions
2
100
Tutorials
Posing of questions and doubts.
Out of class: Posing of questions and
doubts by e-mail and via virtual
classroom
9
50
Student work: study or individual or group work
Preparation of an assignment for
presentation in class
Students work:
Individual studying of basic concepts and methods
69
0
Individual test (oral or written)
Official individual written examination consisting of two questions for each teaching unit
The final grade will be calculated as the arithmetic mean of the grades obtained in the six teaching units.
To pass the examination, a minimum grade of 4.0 points is required.
65 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Individual oral presentation in class of a research article during a maximum of 15 minutes with 5 minutes of questions.
the students will synthesize their knowledge to a subject of mutual agreement with the
teacher.
Assessment will be based on the established rubrics for the following criteria: quality and rigor of the content, organization of information, clarity of presentation, oral communication skills, adherence to the allocated time, and ability to respond to questions.
To pass the assessment, a minimum grade of 6.0 points is required.
35 %
Author: Núñez, Rafael
Title: Flow cytometry for research scientists principles and applications
Editorial: Horizon
Publication Date: 2001
ISBN: 1898486263
Author:
Title: Genome sequencing technology and algorithms
Editorial: Artech House,
Publication Date: 2008
ISBN: 9781596930940
Author: Brown, T.A.
Title: Genomes 3
Editorial: Wiley-Liss
Publication Date: 2007
ISBN: 0815341385
Author: Cserháti, Tibor
Title: Chromatography in food science and technology
Editorial: Technomic
Publication Date: 1999
ISBN: 1566767490
Author: Grimstone, Albert Victor
Title: El microscopio electrónico en Biología /
Editorial: Omega,
Publication Date: 1981
ISBN:
Author: Skoog, Douglas A.
Title: Principios de análisis instrumental
Editorial: McGraw-Hill
Publication Date: 2018
ISBN: 9786075266558
Author: Westermeier, Reiner,
Title: Electrophoresis in practice: a guide to methods and applications of DNA and protein separations /
Editorial:
Publication Date:
ISBN: 9783527695188
Author: Miguel Aballe Caride
Title: Microscopía electrónica de barrido y microanálisis por rayos X
Editorial: Consejo Superior de Investigaciones Científicas
Publication Date: 1996
ISBN: 8472070948, 9788472070943
Author: Goldstein et al.
Title: Scanning Electron Microscopy and X-Ray
Editorial: Springer
Publication Date: 2018
ISBN: 978-1-4939-6676-9
Author: Biassoni R., Raso A.
Title: Quantitative Real-Time PCR
Editorial: Humana
Publication Date: 2019
ISBN: 978-1493998326
Author: Dashek, William V.
Title: Methods in plant biochemistry and molecular biology
Editorial: CRC
Publication Date: 1997
ISBN: 9781315895383
Author: Izquierdo Rojo, Marta
Title: Ingeniería genética y transferencia génica
Editorial: Pirámide
Publication Date: 2001
ISBN: 8436815637
Author: Kader, Adel A.
Title: Postharvest technology of horticultural crops
Editorial: University of California
Publication Date: 2002
ISBN: 1879906511
Author: Glick, Bernard R.
Title: Molecular biotechnology: principles and applications of recombinant DNA
Editorial: ASM Press
Publication Date: 2017
ISBN: 9781555819361
Unidad didáctica 1:
High performance liquid chromatography: A users guide
http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html
http://ull.chemistry.uakron.edu/chemsep/gc/
http://www.shsu.edu/~chm_tgc/sounds/sound.html
http://www.netaccess.on.ca/~dbc/cic_hamilton/liquid.html
Unidad didáctica 2:
Chromatography network. http://www.chromatographynetwork.com
http://www.uib.es/depart/dqu/dquo/pau/Cromatograf%92a/chrom10/chrom/GC/con
cept/main.htm
Chromatography hazards.
http://129.93.84.115/Chemistry/DoChem/DoChem128.html#Hazards
Ballester, P. 2000. Cromatografía de gases. Version 2 Julio 2003. University of Richmond. UIB.
Banks, N.H., Cleland, D.J., Cameron, A.C., Beaudry, R.M., Kader, A.A. 1995. Proposal for a rationalized system of units for postharvest research in gas exchange. HortScience 30: 1129-1131.
Kader, A.A. 1992. Methods of gas mixing, sampling and analysis. En: Kader, A.A. (ed.) Postharvest Technology of Horticultural Crops. Publication No. 3311.Division of Agriculture and Natural Resources, University of California, CA. pp. 93-96.
Saltveit, M.E. 2003. Measuring respiration. http://www.ucdavis.edu 5 pp.
Unidad didáctica 6:
http://www.ornl.gov/sci/techresources/Human_Genome/faq/seqfacts.shtml
http://biotech.about.com/od/pcr/a/sequencing.htm
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.dnalc.org/resources/animations/pcr.html
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.gene-quantification.de/hrm.html
http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html
Morozova, O. & Marra, M.A. (2008). Applications of next-generation sequencing technologies in functional genomics. Genomics 92: 255-264.
Mullis, K.B. (1990). Reacción en cadena de la polimerasa. Investigación y Ciencia 165: 30-47.
Morozova, O. & Marra, M.A. (2008). Applications of next-generation sequencing
Technologies in functional genomics. Genomics 92: 255-264.
Name: TECHNIQUES IN CELLULAR AND MOLECULAR BIOLOGY
Code: 203401001
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 1st Year - First term
Speciality: Especialidad en Tecnología de Alimentos y Biotecnología Agroalimentaria
Language: English
Mode of study: Semi on-site class
Lecturer data: WEISS , JULIA ROSL
Knowledge area: Genética
Department: Ingeniería Agronómica
Telephone: 968325777
Email: julia.weiss@upct.es
Office hours and location:
miércoles - 13:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.15
Se ruega contactar previamente por correo electrónico indicando emotivo de la tutoría.
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 5
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G1, G2
Lecturer data: WEISS , JULIA ROSL
Knowledge area: Genética
Department: Ingeniería Agronómica
Telephone: 968325777
Email: julia.weiss@upct.es
Office hours and location:
miércoles - 13:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.15
Se ruega contactar previamente por correo electrónico indicando emotivo de la tutoría.
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 5
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G1, G2
Lecturer data: PALOP GÓMEZ, ALFREDO
Knowledge area: Tecnología de Alimentos
Department: Ingeniería Agronómica
Telephone: 968325762
Email: alfredo.palop@upct.es
Office hours and location: Tutorials will be carried out by request of the student sending an email to alfredo.palop@upct.es
Qualifications/Degrees:
PhD in Veterinary from University of Zaragoza (SPAIN) - 1995
Academic rank in UPCT: Catedrático de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: PALOP GÓMEZ, ALFREDO
Knowledge area: Tecnología de Alimentos
Department: Ingeniería Agronómica
Telephone: 968325762
Email: alfredo.palop@upct.es
Office hours and location: Tutorials will be carried out by request of the student sending an email to alfredo.palop@upct.es
Qualifications/Degrees:
PhD in Veterinary from University of Zaragoza (SPAIN) - 1995
Academic rank in UPCT: Catedrático de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
1.Identify different DNA analysis techniques, including sequencing and melting point analysis.
2.Understand the technique of scanning electron microscopy (SEM).
3.Understand the technique of gas chromatography¿mass spectrometry (GC¿MS).
4.Understand how high-performance liquid chromatography (HPLC) works.
5.Understand the technique of flow cytometry.
6.Understand the technique of protein electrophoresis.
1.Describe the basic concepts of DNA sequencing, including Sanger sequencing and Next-Generation Sequencing (NGS).
2.Explain the basic principles of scanning electron microscopy (SEM).
3.Identify the basic principles of metabolite separation by high-performance liquid chromatography (HPLC).
4.List the operation and applications of flow cytometry in the agri-food sector.
5.Identify the basic principles of proteomics and protein separation.
6.Explain the operation of gas chromatography¿mass spectrometry (GC¿MS) and its applications in the agri-food industry.
1. Sanger DNA Sequencing and Next-Generation Sequencing (NGS). 2. Scanning Electron Microscopy (SEM). 3. High-Performance Liquid Chromatography (HPLC). 4. Flow Cytometry. 5. Proteomics and Protein Separation. 6. Gas Chromatography¿Mass Spectrometry (GC¿MS).
Teaching unit 1 - Analysis of metabolites by HPLC
1.1.Preparation of samples for HPLC analysis
1.2.Components and methods in HPLC operations
1.3.Application of HPLC technique
1.4.Guide for problems that can occur during HPLC operation
Teaching unit 2 - Analysis of metabolites by gas chromatography
2.1.Introduction to chromatography
Definition
Basic principles
Components of a gas chromatograph, hazards and critical control points.
Identification of compounds, quantification
Recent innovations
2.2.Application in the agro-alimentary field (composition, no exogenous, foreign elements)
Simple gases and headspace with and without micro extraction in solid phase and/or
derivatisation.
Other applications: Purge and trap, electronic nose, non-destructive systems, stir bar sorptive
extraction, headspace aroma fingerprinting, static and dynamic (continuous) systems.
Examples in the agro-alimentary field and extraction and analysis of volatile aromas from plant
tissues.
Teaching unit 3 - Analysis of proteins by mono ¿ and bi-dimensional electrophoresis
3.1. Introduction. Basic principles of electrophoresis
3.2. Electrophoretic techniques
Polyacrylamide gel electrophoresis: native, SDS and isoelectric focusing.
Bi-dimensional electrophoresis. Preparation of samples. First dimension. Second dimension.
Protein detection. Proteomic analysis.
Teaching unit 4 - Flow cytometry
4.1 Fundamentals of the technique
4.2 Optic and electronic of a flow cytometer
4.3 Cell sorting
4.4 Applications and practical examples.
Teaching unit 5 - The scanning electron microscope
5.1 Main concepts of the technique
5.2 Structure and functioning
5.2 Sample preparation
5.3 Applications, advantages and disadvantages
Teaching unit 6 - DNA sequence analysis
6.1 Introduction to DNA sequence analysis
6.2 Basic molecular techniques with relevance for sequencing and melting peak analysis.
6.3 Sequencing methods ¿ Sanger; pyrosequencing; next generation sequencing.
6.4 DNA sequence analysis by melting peak analysis and high resolution melting analysis.
6.5 Applications
Teaching unit 1
- Extraction and analysis of metabolites by HPLC
Teaching unit 2
- Extraction and analysis of metabolites by gas chromatography
Teaching unit 3
- Analysis of proteins by mono- and bi-dimensional electrophoresis
Teaching unit 5
- Basic handling of the scanning electron microscope
Teaching unit 6
- Basic handling and applications of a thermocycler for real-time PC and high resolution melting analysis
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual") under the "Act in an Emergency" section, "Technical Guides" tab, with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find, in the "Act in an Emergency", sectionrecommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Teaching technique:
Basic program
Presentation of the lecture.
Preparation and provision of
documentation to the virtual
classroom
Resolve queries from students
Students work:
In the class:
Assistance
Personal notes on the documentation
received. Posing of questions and
doubts.
26
0
Class in laboratory: practical classes / internships
Teaching Technique:
Presentation and direction
Students work:
Assistance, development and
presentation
10
100
Continuous assessment activities during class hours.
Theoretical exam in proportion to
each thematic unit of the subject.
Course work (15 minutes / student).
Attendance at the presentation of all
students with discussion and
questions.
4
100
Final and ongoing assessment activities outside class hours.
Theoretical exam in proportion to
each thematic unit of the subject.
Course work (15 minutes / student).
Attendance at the presentation of all
students with discussion and
questions
2
100
Tutorials
Posing of questions and doubts.
Out of class: Posing of questions and
doubts by e-mail and via virtual
classroom
9
50
Student work: study or individual or group work
Preparation of an assignment for
presentation in class
Students work:
Individual studying of basic concepts and methods
69
0
Individual test (oral or written)
Official individual written examination consisting of two questions for each teaching unit
The final grade will be calculated as the arithmetic mean of the grades obtained in the six teaching units.
To pass the examination, a minimum grade of 4.0 points is required.
65 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Individual oral presentation in class of a research article during a maximum of 15 minutes with 5 minutes of questions.
the students will synthesize their knowledge to a subject of mutual agreement with the
teacher.
Assessment will be based on the established rubrics for the following criteria: quality and rigor of the content, organization of information, clarity of presentation, oral communication skills, adherence to the allocated time, and ability to respond to questions.
To pass the assessment, a minimum grade of 6.0 points is required.
35 %
Author: Núñez, Rafael
Title: Flow cytometry for research scientists principles and applications
Editorial: Horizon
Publication Date: 2001
ISBN: 1898486263
Author:
Title: Genome sequencing technology and algorithms
Editorial: Artech House,
Publication Date: 2008
ISBN: 9781596930940
Author: Brown, T.A.
Title: Genomes 3
Editorial: Wiley-Liss
Publication Date: 2007
ISBN: 0815341385
Author: Cserháti, Tibor
Title: Chromatography in food science and technology
Editorial: Technomic
Publication Date: 1999
ISBN: 1566767490
Author: Grimstone, Albert Victor
Title: El microscopio electrónico en Biología /
Editorial: Omega,
Publication Date: 1981
ISBN:
Author: Skoog, Douglas A.
Title: Principios de análisis instrumental
Editorial: McGraw-Hill
Publication Date: 2018
ISBN: 9786075266558
Author: Westermeier, Reiner,
Title: Electrophoresis in practice: a guide to methods and applications of DNA and protein separations /
Editorial:
Publication Date:
ISBN: 9783527695188
Author: Miguel Aballe Caride
Title: Microscopía electrónica de barrido y microanálisis por rayos X
Editorial: Consejo Superior de Investigaciones Científicas
Publication Date: 1996
ISBN: 8472070948, 9788472070943
Author: Goldstein et al.
Title: Scanning Electron Microscopy and X-Ray
Editorial: Springer
Publication Date: 2018
ISBN: 978-1-4939-6676-9
Author: Biassoni R., Raso A.
Title: Quantitative Real-Time PCR
Editorial: Humana
Publication Date: 2019
ISBN: 978-1493998326
Author: Dashek, William V.
Title: Methods in plant biochemistry and molecular biology
Editorial: CRC
Publication Date: 1997
ISBN: 9781315895383
Author: Izquierdo Rojo, Marta
Title: Ingeniería genética y transferencia génica
Editorial: Pirámide
Publication Date: 2001
ISBN: 8436815637
Author: Kader, Adel A.
Title: Postharvest technology of horticultural crops
Editorial: University of California
Publication Date: 2002
ISBN: 1879906511
Author: Glick, Bernard R.
Title: Molecular biotechnology: principles and applications of recombinant DNA
Editorial: ASM Press
Publication Date: 2017
ISBN: 9781555819361
Unidad didáctica 1:
High performance liquid chromatography: A users guide
http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html
http://ull.chemistry.uakron.edu/chemsep/gc/
http://www.shsu.edu/~chm_tgc/sounds/sound.html
http://www.netaccess.on.ca/~dbc/cic_hamilton/liquid.html
Unidad didáctica 2:
Chromatography network. http://www.chromatographynetwork.com
http://www.uib.es/depart/dqu/dquo/pau/Cromatograf%92a/chrom10/chrom/GC/con
cept/main.htm
Chromatography hazards.
http://129.93.84.115/Chemistry/DoChem/DoChem128.html#Hazards
Ballester, P. 2000. Cromatografía de gases. Version 2 Julio 2003. University of Richmond. UIB.
Banks, N.H., Cleland, D.J., Cameron, A.C., Beaudry, R.M., Kader, A.A. 1995. Proposal for a rationalized system of units for postharvest research in gas exchange. HortScience 30: 1129-1131.
Kader, A.A. 1992. Methods of gas mixing, sampling and analysis. En: Kader, A.A. (ed.) Postharvest Technology of Horticultural Crops. Publication No. 3311.Division of Agriculture and Natural Resources, University of California, CA. pp. 93-96.
Saltveit, M.E. 2003. Measuring respiration. http://www.ucdavis.edu 5 pp.
Unidad didáctica 6:
http://www.ornl.gov/sci/techresources/Human_Genome/faq/seqfacts.shtml
http://biotech.about.com/od/pcr/a/sequencing.htm
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.dnalc.org/resources/animations/pcr.html
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.gene-quantification.de/hrm.html
http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html
Morozova, O. & Marra, M.A. (2008). Applications of next-generation sequencing technologies in functional genomics. Genomics 92: 255-264.
Mullis, K.B. (1990). Reacción en cadena de la polimerasa. Investigación y Ciencia 165: 30-47.
Morozova, O. & Marra, M.A. (2008). Applications of next-generation sequencing
Technologies in functional genomics. Genomics 92: 255-264.