Name: ADVANCED TECHNIQUES IN VITRO PLANT CULTURE: MICROPROPAGATION AND PRODUCTION OF VALUABLE COMPOUNDS
Code: 203401003
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 1st Year - First term
Speciality: Especialidad en Tecnología de Alimentos y Biotecnología Agroalimentaria
Language: English
Mode of study: On-site class
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G2
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G2
Lecturer data: CALDERÓN GARCÍA, ANTONIO ASENSIO
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325542
Email: antonio.calderon@upct.es
Office hours and location:
martes - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
miércoles - 16:00 / 18:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
jueves - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
Qualifications/Degrees:
Academic rank in UPCT: Profesor Titular de Universidad
Number of five-year periods: 7
Number of six-year periods: 5 de investigación
Curriculum Vitae: Full Profile
Lecturer data: CALDERÓN GARCÍA, ANTONIO ASENSIO
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325542
Email: antonio.calderon@upct.es
Office hours and location:
martes - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
miércoles - 16:00 / 18:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
jueves - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
Qualifications/Degrees:
Academic rank in UPCT: Profesor Titular de Universidad
Number of five-year periods: 7
Number of six-year periods: 5 de investigación
Curriculum Vitae: Full Profile
1. Interpret, critically discuss, and orally communicate research articles on the subject.
2. Identify design errors and propose solutions within facilities dedicated to the in vitro culture of plant material.
3. Master the techniques used in the in vitro culture of plant material for the purpose of propagating and conserving it, as well as obtaining metabolites of industrial interest.
1. Understand the factors that determine the responses of plant material cultured in vitro and how to modify them.
2. Analyze, explain, and discuss the characteristics of facilities and processes used in in vitro culture.
3. Propose new processes, or improvements to existing ones, to optimize and increase the profitability of plant production and/or the production of metabolites from plant material cultured in vitro.
1. Facilities and equipment of a micropropagation laboratory. 2. Micropropagation. Automation and large-scale cultivation. 3. Limitations of plant micropropagation. 4. Problems during establishment and cultivation: Contamination, hypersensitive reactions, waterlogging, heterotrophy. 5. Problems following transfer to ex vitro conditions. 6. Applications of micropropagation techniques. 7. Economic impact of micropropagation. Major producers and the market for micropropagated plants. 8. Introduction to the study of the biosynthetic potential of plants. Industrial utilization. 9. Special techniques for metabolite production: Addition of precursors, biotransformation, elicitation, mixed cultures. 10. Industrial-scale cultures: Types of bioreactors, operation of bioreactors. 11. Applications: Production of dyes, antioxidants, and other products of pharmaceutical interest. Production of biopolymers. Production of proteins. Antibodies.
Unit 1: Introduction and requeriments
C1. Introduction
C2. Facilities and equipment in a micropropagation laboratory
C3. Aseptic work conditions
Unit 2: Micropropagation: methods, limitacions and aplications
C4. Micropropagation steps
C5. Multiplication methods
C6. Automation and large-scale cultures
C7. Plant micropropagation limitations
C8. Common problems associated with micropropagation
C9. Applications
Unit 3: Biosynthetic potential of plants
C10. Introduction to the study of the plant¿s biosynthetic potential
C11. In vitro culture of plant cells, tissues and organs
C12. Special productions techniques: addition of precursors, elicitation, biotransformation, mixed cultures
C13. Industrial scale cultures
C14. Applications
Unit 1-3
Practice 1 (P1). Construction of a bioreactor for the micropropagation of plant material and monitoring of crops (4 h). Practice 2 (P2). Elicitation of cell cultures and analysis of the production of bioactive compounds (4 h). Practice 3 (P3). Establishment of root crops for the production of secondary metabolites (2 h).
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual") under the "Act in an Emergency" section, "Technical Guides" tab, with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find, in the "Act in an Emergency", sectionrecommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Presentation of the lecture. Resolve queries from students. To develop theoretical knowledge based on concepts and theories
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving
exercises, or practical cases
24
100
Class in laboratory: practical classes / internships
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
10
100
Class in the field or open classroom (technical visits, lectures, etc.).
Visit a company or research centre engaged in plant cell in vitro culture
2
100
Continuous assessment activities during class hours.
Continuous assessment system
4
100
Final and ongoing assessment activities outside class hours.
Attendance to the exams
2
100
Tutorials
Making suggestions for improvement of student work. Resolve doubts.
9
50
Student work: study or individual or group work
Preparation oral exposition
Preparation a formal lab report.
Preparation oral exposition.
Literature search
Self-study: Study the lectures
69
0
Individual test (oral or written)
Individual test (oral or written)
Written exam mainly assessing theoretical concepts, consisting of multiple choice questions and short questions. The exam will take place on the date and time established by the coordinator. The written theory assessment will have a corresponding assessment activity in the ordinary and extraordinary examination periods.
25 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the rubric. The laboratory practical assessment will also have an equivalent assessment activity available in both ordinary and extraordinary examination periods.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions. All the material and details of this exam will be posted in the aula virtual of the subject.
15 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Oral exposition assessment will be done taking into account the criteria established previously in the rubric.
Presentations will be scheduled during the teaching period, once the theoretical classes have been completed.
60 %
The course will be assessed through continuous assessment (1), the ordinary examination session (2), and the extraordinary examination session (3).
Course assessment is based on three main components: "Laboratory practical assessment" (15%), "Theory assessment" (25%), and "Presentation and defence of individual work" (60%). These three activities within the continuous assessment system will be carried out during the teaching period.
Ordinary and extraordinary assessment periods will include equivalent assessment activities allowing students to obtain up to 100% of the final grade. The written exam will assess the theoretical component (25%). A practical examination or equivalent task will assess laboratory competences (15%). An oral presentation and defence or an equivalent individual assignment with oral defence will assess the presentation component (60%). Students will only need to retake the activities not previously passed.
Author: Altman, A.
Title: Agricultural biotechnology
Editorial: Marcel Dekker
Publication Date: 1998
ISBN: 0824794397
Author: Davey, Michael R.
Title: Plant cell culture essential methods
Editorial: Wiley-Blackwell,
Publication Date: 2010
ISBN: 9780470686485
Author:
Title: Plant tissue culture engineering
Editorial: Springer
Publication Date: 2006
ISBN: 9781402035944
Aula virtual (several research articles related with the different topics of this subject will be made available on the aula virtual)
http://plant-tc.cfans.umn.edu/listserv/
Name: ADVANCED TECHNIQUES IN VITRO PLANT CULTURE: MICROPROPAGATION AND PRODUCTION OF VALUABLE COMPOUNDS
Code: 203401003
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 1st Year - First term
Speciality: Especialidad en Tecnología de Alimentos y Biotecnología Agroalimentaria
Language: English
Mode of study: Semi on-site class
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G2
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G2
Lecturer data: CALDERÓN GARCÍA, ANTONIO ASENSIO
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325542
Email: antonio.calderon@upct.es
Office hours and location:
martes - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
miércoles - 16:00 / 18:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
jueves - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
Qualifications/Degrees:
Academic rank in UPCT: Profesor Titular de Universidad
Number of five-year periods: 7
Number of six-year periods: 5 de investigación
Curriculum Vitae: Full Profile
Lecturer data: CALDERÓN GARCÍA, ANTONIO ASENSIO
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325542
Email: antonio.calderon@upct.es
Office hours and location:
martes - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
miércoles - 16:00 / 18:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
jueves - 12:00 / 14:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.17
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados
Qualifications/Degrees:
Academic rank in UPCT: Profesor Titular de Universidad
Number of five-year periods: 7
Number of six-year periods: 5 de investigación
Curriculum Vitae: Full Profile
1. Interpret, critically discuss, and orally communicate research articles on the subject.
2. Identify design errors and propose solutions within facilities dedicated to the in vitro culture of plant material.
3. Master the techniques used in the in vitro culture of plant material for the purpose of propagating and conserving it, as well as obtaining metabolites of industrial interest.
1. Understand the factors that determine the responses of plant material cultured in vitro and how to modify them.
2. Analyze, explain, and discuss the characteristics of facilities and processes used in in vitro culture.
3. Propose new processes, or improvements to existing ones, to optimize and increase the profitability of plant production and/or the production of metabolites from plant material cultured in vitro.
1. Facilities and equipment of a micropropagation laboratory. 2. Micropropagation. Automation and large-scale cultivation. 3. Limitations of plant micropropagation. 4. Problems during establishment and cultivation: Contamination, hypersensitive reactions, waterlogging, heterotrophy. 5. Problems following transfer to ex vitro conditions. 6. Applications of micropropagation techniques. 7. Economic impact of micropropagation. Major producers and the market for micropropagated plants. 8. Introduction to the study of the biosynthetic potential of plants. Industrial utilization. 9. Special techniques for metabolite production: Addition of precursors, biotransformation, elicitation, mixed cultures. 10. Industrial-scale cultures: Types of bioreactors, operation of bioreactors. 11. Applications: Production of dyes, antioxidants, and other products of pharmaceutical interest. Production of biopolymers. Production of proteins. Antibodies.
Unit 1: Introduction and requeriments
C1. Introduction
C2. Facilities and equipment in a micropropagation laboratory
C3. Aseptic work conditions
Unit 2: Micropropagation: methods, limitacions and aplications
C4. Micropropagation steps
C5. Multiplication methods
C6. Automation and large-scale cultures
C7. Plant micropropagation limitations
C8. Common problems associated with micropropagation
C9. Applications
Unit 3: Biosynthetic potential of plants
C10. Introduction to the study of the plant¿s biosynthetic potential
C11. In vitro culture of plant cells, tissues and organs
C12. Special productions techniques: addition of precursors, elicitation, biotransformation, mixed cultures
C13. Industrial scale cultures
C14. Applications
Unit 1-3
Practice 1 (P1). Construction of a bioreactor for the micropropagation of plant material and monitoring of crops (4 h). Practice 2 (P2). Elicitation of cell cultures and analysis of the production of bioactive compounds (4 h). Practice 3 (P3). Establishment of root crops for the production of secondary metabolites (2 h).
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual") under the "Act in an Emergency" section, "Technical Guides" tab, with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find, in the "Act in an Emergency", sectionrecommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Presentation of the lecture. Resolve queries from students. To develop theoretical knowledge based on concepts and theories
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving
exercises, or practical cases
24
0
Class in laboratory: practical classes / internships
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
10
100
Class in the field or open classroom (technical visits, lectures, etc.).
Visit a company or research centre engaged in plant cell in vitro culture
2
100
Continuous assessment activities during class hours.
Continuous assessment system
4
100
Final and ongoing assessment activities outside class hours.
Attendance to the exams
2
100
Tutorials
Making suggestions for improvement of student work. Resolve doubts.
9
50
Student work: study or individual or group work
Preparation oral exposition
Preparation a formal lab report.
Preparation oral exposition.
Literature search
Self-study: Study the lectures
69
0
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions.
The exam will take place on the day and time established by the coordinator.
25 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
15 %
Presentation and defence of individual or group assignments (it may include auto-evaluation and peer-evaluation)
Oral exposition assessment will be done taking into account the criteria established previously in the rubric. Presentations will be scheduled during the teaching period, once the theoretical classes have been completed.
60 %
The course will be assessed through continuous assessment (1), the ordinary examination session (2), and the extraordinary examination session (3).
Course assessment is based on three main components: Laboratory practical assessment (15%), Theory assessment (25%), and Presentation and defence of individual work (60%). These three activities within the continuous assessment system will be carried out during the teaching period.
In both the ordinary and extraordinary examination sessions, students may retake any components not passed through continuous assessment. Grades for components that have been passed will be retained throughout the academic year. The weighting of assessment components will remain the same as in the continuous assessment system.
Author: Altman, A.
Title: Agricultural biotechnology
Editorial: Marcel Dekker
Publication Date: 1998
ISBN: 0824794397
Author: Davey, Michael R.
Title: Plant cell culture essential methods
Editorial: Wiley-Blackwell,
Publication Date: 2010
ISBN: 9780470686485
Author:
Title: Plant tissue culture engineering
Editorial: Springer
Publication Date: 2006
ISBN: 9781402035944
Aula virtual (several research articles related with the different topics of this subject will be made available on the aula virtual)
http://plant-tc.cfans.umn.edu/listserv/