Name: TECHNIQUES IN CELLULAR AND MOLECULAR BIOLOGY
Code: 229102004
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 2nd Year - First term
Speciality:
Language: English
Mode of study: On-site class
Lecturer data: WEISS , JULIA ROSL
Knowledge area: Genética
Department: Ingeniería Agronómica
Telephone: 968325777
Email: julia.weiss@upct.es
Office hours and location:
miércoles - 13:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.15
Se ruega contactar previamente por correo electrónico indicando emotivo de la tutoría.
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 4
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: PALOP GÓMEZ, ALFREDO
Knowledge area: Tecnología de Alimentos
Department: Ingeniería Agronómica
Telephone: 968325762
Email: alfredo.palop@upct.es
Office hours and location:
lunes - 10:00 / 10:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.25
Para cualquier tutoría, contactar previamente por correo electrónico
Qualifications/Degrees:
PhD in Veterinary from University of Zaragoza (SPAIN) - 1995
Academic rank in UPCT: Catedrático de Universidad
Number of five-year periods: 5
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Se trata de una asignatura optativa de la titulación pero que tiene carácter obligatorio si se elige cursar el módulo optativo de formación investigadora. Las competencias de dicho Módulo a adquirir son:
Técnicas de Biología Celular y Molecular: Conocimientos a nivel de usuario principiante de técnicas de secuenciación, análisis de polimorfismos de ADN por punto de fusión, microscopía electrónica de barrido, cromatografía de gases-espectrometría de masas, HPLC y electroforésis de proteínas
Herramientas de genómica en investigación: Conocimientos básicos de estructura de genomas y métodos de análisis. Conocimientos a nivel de usuario principiante de programas bioinformáticos para búsqueda de secuencias en bases de datos. Programas Clustal X, BLAST y NJPLOT
Diseño de experimentos en investigación agraria y alimentaria: Plantear de forma autónoma experimentos (problemas e hipótesis). Analizar resultados para aceptar o rechazar hipótesis de partida e inferir conclusiones.
Técnicas de Biología Celular y Molecular
1.- Conocer los conceptos básicos sobre la secuenciación de ADN en el ámbito de secuenciación Sanger y Next-Generation Sequencing.
2.- Entender los conceptos básicos de la microscopía electrónica de barrido.
3.- Entender los principios básicos de la separación de metabolitos por HPLC.
4.- Conocer el funcionamiento y utilidad de la citometría de flujo en el ámbito agroalimentario.
5.- Aprender los principios básicos de la proteómica y separación de proteínas.
6.- GC-MS y sus aplicaciones en la industria agroalimentaria.
Herramientas de genómica en investigación
1.- Conocimiento de la estructura de los genomas
2.- Entender las técnicas de estudio de genómica estructural
3.- Conocimiento del funcionamiento del genoma
4.- Conocer las técnicas de análisis de transcriptomas
5.- Aprender las metodologías utilizadas para la obtención de ganancia y pérdida de función de genes.
6.- Tener conocimientos básicos de uso de programas BLAST, CLUSTALX y NJPLOT
Diseño de experimentos en investigación agraria y alimentaria
1.- Enseñar cómo platear hipótesis y diseñar un experimento científico.
2.- Familiarizar al alumno con los diferentes tipos de diseños, especialmente aquellos más comunes en agricultura y alimentación.
3.- Describir los modelos matemáticos para analizar los resultados de cada diseño, y finalmente aceptar o rechazar la hipótesis de partida.
4.- Dar una visión global de las limitaciones y capacidades de inferir conclusiones de los resultados de uno varios experimentos.
Técnicas de Biología Celular y Molecular 1. Secuenciación de ADN (Sanger y Next-Generation Sequencing). 2. Microscopía electrónica de barrido. 3. Separación de metabolitos por HPLC. 4. Citometría de flujo en el ámbito agroalimentario. 5. Proteómica y separación de proteínas. 6. GC-MS y sus aplicaciones en la industria agroalimentaria. Herramientas de genómica en investigación 1. Estructura de los genomas 2. Técnicas de estudio de genómica estructural 3. Funcionamiento del genoma 4. Técnicas de análisis de transcriptomas 5. Metodologías para la obtención de ganancia y pérdida de función de genes. 6. Uso de programas BLAST, CLUSTALX y NJPLOT Diseño de experimentos en investigación agraria y alimentaria 1. introducción 2. estadística descriptiva 3. probabilidad y distribuciones 4. test de hipótesis 5. introducción al análisis de varianza 6. análisis de varianza con un factor 7. análisis de varianza con factores múltiples 8. supuestos del análisis de varianza 9. separación de medias en grupos homogéneos 10. Regresión lineal 11. Correlación 12. Regresión múltiple 13. Análisis de proporciones 14. Estadística no paramétrica
Teaching unit 1 - Analysis of metabolites by HPLC
1.1.Preparation of samples for HPLC analysis
1.2.Components and methods in HPLC operations
1.3.Application of HPLC technique
1.4.Guide for problems that can occur during HPLC operation
Teaching unit 2 - Analysis of metabolites by gas chromatography
2.1.Introduction to chromatography
Definition
Basic principles
Components of a gas chromatograph, hazards and critical control points.
Identification of compounds, quantification
Recent innovations
2.2.Application in the agro-alimentary field (composition, no exogenous, foreign elements)
Simple gases and headspace with and without micro extraction in solid phase and/or derivatisation.
Other applications: Purge and trap, electronic nose, non-destructive systems, stir bar sorptive extraction, headspace aroma fingerprinting, static and dynamic (continuous) systems.
Examples in the agro-alimentary field and extraction and analysis of volatile aromas from plant tissues.
Teaching unit 3 - Analysis of proteins by mono ¿ and bi-dimensional electrophoresis
3.1. Introduction. Basic principles of electrophoresis
3.2. Electrophoretic techniques
Polyacrylamide gel electrophoresis: native, SDS and isoelectric focusing.
Bi-dimensional electrophoresis. Preparation of samples. First dimension. Second dimension. Protein detection. Proteomic analysis.
Teaching unit 4 - Flow cytometry
4.1 Fundamentals of the technique
4.2 Optic and electronic of a flow cytometer
4.3 Cell sorting
4.4 Applications and practical examples.
Teaching unit 5 - The scanning electron microscope
5.1 Main concepts of the technique
5.2 Structure and functioning
5.2 Sample preparation
5.3 Applications, advantages and disadvantages
Teaching unit 6 - DNA sequence analysis
6.1 Introduction to DNA sequence analysis
6.2 Basic molecular techniques with relevance for sequencing and melting peak analysis.
6.3 Sequencing methods ¿ Sanger; pyrosequencing; next generation sequencing.
6.4 DNA sequence analysis by melting peak analysis and high resolution melting analysis.
6.5 Applications
Teaching unit 1
- Extraction and analysis of metabolites by HPLC
Teaching unit 2
- Extraction and analysis of metabolites by gas chromatography
Teaching unit 3
- Analysis of proteins by mono- and bi-dimensional electrophoresis
Teaching unit 5
- Basic handling of the scanning electron microscope
Teaching unit 6
- Basic handling and applications of a next generation sequencing device and a thermocycler for real-time PCR
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Teaching technique:
Basic program
Presentation of the lecture.
Preparation and provision of documentation to the virtual classroom
Resolve queries from students
Students work:
In the class:
Assistance
Personal notes on the documentation received. Posing of questions and doubts.
26
100
Class in laboratory: practical classes / internships
Teaching Technique:
Presentation and direction
Students work:
Assistance, development and presentation
10
100
Class in the field or open classroom (technical visits, lectures, etc.). In general, activities that require special resources or planning
Teaching Technique:
Presentation and direction
Students work:
Assistance, development and presentation
0
100
Class in a computer classroom: practical classes / internships
0
100
Assessment activities (continuous assessment system)
Theoretical exam in proportion to each thematic unit of the subject.
Course work (15 minutes / student). Attendance at the presentation of all students with discussion and questions.
4
100
Assessment activities (final assessment system)
Theoretical exam in proportion to each thematic unit of the subject.
Course work (15 minutes / student). Attendance at the presentation of all students with discussion and questions.
4
100
Tutorials
Posing of questions and doubts.
Out of class: Posing of questions and doubts by e-mail and via virtual classroom
4
50
Student work: study or individual or group work
Preparation of an assignment for presentation in class
Students work:
Individual studying of basic concepts and methods
72
0
Individual official test
Written exam; 2 questions / module (6 modules) oriented fundamentally to evaluate the learning of basic concepts.
65 %
Presentation and defence of individual or group assignments
Presentation, during a maximum of 15 minutes, with 5 minutes of questions, of a work. In this work, the student will synthesize their knowledge to a subject of mutual agreement with the teacher.
35 %
Individual official test
Written exam; 2 questions / module (6 modules) oriented fundamentally to evaluate the learning of basic concepts.
65 %
Presentation and defence of individual or group assignments
Presentation, during a maximum of 15 minutes, with 5 minutes of questions, of a work. In this work, the student will synthesize their knowledge to a subject of mutual agreement with the teacher.
35 %
Performance of small tests of short duration in class
Author: Glick, Bernard R.
Title: Molecular biotechnology: principles and applications of recombinant DNA
Editorial: ASM press
Publication Date: 1998
ISBN: 1555811361
Author: Dashek, William V.
Title: Methods in plant biochemistry and molecular biology
Editorial: CRC
Publication Date: 1997
ISBN: 9781315895383
Author: Núñez, Rafael
Title: Flow cytometry for research scientists principles and applications
Editorial: Horizon
Publication Date: 2001
ISBN: 1898486263
Author:
Title: Genome sequencing technology and algorithms
Editorial: Artech House,
Publication Date: 2008
ISBN: 9781596930940
Author: Aballe Caride, Miguel
Title: Microscopía electrónica de barrido y microanálisis por rayos X
Editorial: Consejo Superior de Investigaciones Científicas, : Rueda
Publication Date: 1996
ISBN: 8472070944
Author: Brown, T.A.
Title: Genomes 3
Editorial: Wiley-Liss
Publication Date: 2007
ISBN: 0815341385
Author: Cserháti, Tibor
Title: Chromatography in food science and technology
Editorial: Technomic
Publication Date: 1999
ISBN: 1566767490
Author: Grimstone, Albert Victor
Title: El microscopio electrónico en Biología /
Editorial: Omega,
Publication Date: 1981
ISBN:
Author: Skoog, Douglas A.
Title: Principios de análisis instrumental
Editorial: McGraw-Hill
Publication Date: 2018
ISBN: 9786075266558
Author: Orfao, A. et al.
Title: La citometría de flujo en el diagnóstico clínico
Editorial: Servicio General de Citometría. Universidad de Salamanca
Publication Date: 1993
ISBN:
Author: Goldstein et al.
Title: Scanning electrón microscope and X-ray microanálisis.
Editorial: Plenum Press.
Publication Date: 1992
ISBN: 0306441756
Author: Westermeier R.
Title: Electrophoresis in practice
Editorial: 3rd ed. Wiley-VCH.
Publication Date: 2000
ISBN: 978352731181
Author: Biassoni R., Raso A.
Title: Quantitative Real-Time PCR
Editorial: Humana
Publication Date: 2019
ISBN: 978-1493998326
Author: Mullis
Title: Reacción en cadena de la polimerasa.
Editorial: Investigación y Ciencia 165: 30-47.
Publication Date: 1990
ISBN:
Author: Ballester, P.
Title: Cromatografía de gases
Editorial: University of Richmond. UIB.
Publication Date: 2003
ISBN:
Author: Banks, N.H. et al..
Title: Proposal for a rationalized system of units for postharvest research in gas exchange.
Editorial: HortScience 30: 1129-1131.
Publication Date: 1995
ISBN:
Author: Kader, A.A.
Title: Methods of gas mixing, sampling and analysis.
Editorial: Publication No. 3311.Division of Agriculture and Natural Resources, University of California,
Publication Date: 1992
ISBN:
Author: Morozova and Marra
Title: Applications of next-generation sequencing Technologies in functional genomics.
Editorial: Genomics 92: 255-264.
Publication Date: 2008
ISBN:
Unidad didáctica 1:
High performance liquid chromatography: A users guide
http://kerouac.pharm.uky.edu/ASRG/HPLC/hplcmytry.html
http://ull.chemistry.uakron.edu/chemsep/gc/
http://www.shsu.edu/~chm_tgc/sounds/sound.html
http://www.netaccess.on.ca/~dbc/cic_hamilton/liquid.html
Unidad didáctica 2:
Chromatography network. http://www.chromatographynetwork.com
http://www.uib.es/depart/dqu/dquo/pau/Cromatograf%92a/chrom10/chrom/GC/con
cept/main.htm
Chromatography hazards.
http://129.93.84.115/Chemistry/DoChem/DoChem128.html#Hazards
Ballester, P. 2000. Cromatografía de gases. Version 2 Julio 2003. University of Richmond. UIB.
Banks, N.H., Cleland, D.J., Cameron, A.C., Beaudry, R.M., Kader, A.A. 1995. Proposal for a rationalized system of units for postharvest research in gas exchange. HortScience 30: 1129-1131.
Kader, A.A. 1992. Methods of gas mixing, sampling and analysis. En: Kader, A.A. (ed.) Postharvest Technology of Horticultural Crops. Publication No. 3311.Division of Agriculture and Natural Resources, University of California, CA. pp. 93-96.
Saltveit, M.E. 2003. Measuring respiration. http://www.ucdavis.edu 5 pp.
Unidad didáctica 6:
http://www.ornl.gov/sci/techresources/Human_Genome/faq/seqfacts.shtml
http://biotech.about.com/od/pcr/a/sequencing.htm
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.dnalc.org/resources/animations/pcr.html
http://pathmicro.med.sc.edu/pcr/realtime-home.htm
http://www.gene-quantification.de/hrm.html
http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html
Morozova, O. & Marra, M.A. (2008). Applications of next-generation sequencing technologies in functional genomics. Genomics 92: 255-264.
Mullis, K.B. (1990). Reacción en cadena de la polimerasa. Investigación y Ciencia 165: 30-47.
Morozova, O. & Marra, M.A. (2008). Applications of next-generation sequencing
Technologies in functional genomics. Genomics 92: 255-264.