Name: ADVANCED TECHNIQUES IN IN VITRO PLANT CULTURE:MICROPROPAGATION AND PRODUCTION OF VALUABLE COMPOUNDS
Code: 229102010
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 2nd Year - First term
Speciality:
Language: English
Mode of study: On-site class
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 4 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
The main objective of this subject is to present the recent techniques of in vitro cultivation of cells, tissues and plant organs and how they are modified and adapted to increase the profitability of the industrial production processes based on them. In addition, it is intended that students know the current market situation of these types of products and their future prospects.
It is also intended that students become familiar with the manipulation of plant tissues in aseptic conditions and carry out some of the basic operations frequently used in facilities dedicated to the commercial exploitation of this type of techniques.
It is recommended that the student has a good knowledge of Plant Physiology.
At the end of the course the student should be able:
1¿ To describe the factors that determine the responses of plant material grown in vitro and how to modify them.
2- To analyze, explain and discuss the features of facilities and processes dedicated to in vitro culture.
3- To propose new processes or improvements to existing ones, in order to optimize and make profitable the production and conservation of plants and / or the production of metabolites by using in vitro-cultured plant material.
Técnicas avanzadas de cultivo in vitro: micropropagación y producción de compuestos de interés industrial. 1. Introducción. 2. Limitaciones de la micropropagación vegetal. 3. Aplicaciones de las técnicas de micropropagación. 4. Impacto económico de la micropropagación. Principales productores y mercado de las plantas micropropagadas. 5. Introducción al estudio del potencial biosintético de las plantas. Aprovechamiento industrial. 6. Cultivos a escala industrial. 7. Aplicaciones de las técnicas de producción in vitro de metabolitos.
Unit 1: Introduction and requeriments (4 hours)
C1. Introduction
C2. Facilities and equipment in a micropropagation laboratory
C3. Aseptic work conditions
Unit 2: Micropropagation: methods, limitacions and aplications (16 hours)
C4. Micropropagation steps
C5. Multiplication methods
C6. Automation and large-scale cultures
C7. Plant micropropagation limitations
C8. Common problems associated with micropropagation
C9. Applications
Unit 3: Biosynthetic potential of plants (10 hours)
C10. Introduction to the study of the plant¿s biosynthetic potential
C11. In vitro culture of plant cells, tissues and organs
C12. Special productions techniques: addition of precursors, elicitation, biotransformation, mixed cultures
C13. Industrial scale cultures
C14. Applications
Prractice syllabus
Practice 1 (P1). Construction of a bioreactor for the micropropagation of plant material and monitoring of crops (4 h). Practice 2 (P2). Elicitation of cell cultures and analysis of the production of bioactive compounds (4 h). Practice 3 (P3). Establishment of root crops for the production of secondary metabolites (2 h).
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Presentation of the lecture. Resolve queries from students. To develop theoretical knowledge based on concepts and theories
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving exercises, or practical cases
26
100
Class in laboratory: practical classes / internships
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
10
100
Class in the field or open classroom (technical visits, lectures, etc.). In general, activities that require special resources or planning
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
Oral exposition of one topic of interest. Activities to develop theoretical, practical or applied knowledge based on specific topics or views of the profession
0
100
Class in a computer classroom: practical classes / internships
Practical class in the computer room: Activities for the acquisition of certain skills through the use of specific software
0
100
Assessment activities (continuous assessment system)
Continuous assessment system.
4
100
Assessment activities (final assessment system)
Attendance to the exams
4
100
Tutorials
Making suggestions for improvement of student work. Resolve doubts.
3
50
Student work: study or individual or group work
Preparation oral exposition
Preparation a formal lab report. Preparation oral exposition. Literature search
Self-study: Study the lectures
73
0
Individual official test
Multiple choice questions and short questions
30 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions. All the material and details of this exam will be posted in the aula virtual of the subject.
20 %
Presentation and defence of individual or group assignments
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
45 %
Other evaluation activities
Students will be asked to make a self-assessment report of their oral presentation, as well as an evaluation of the oral exposure of all their classmates.
5 %
Individual official test
Multiple choice questions and short questions
35 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions. All the material and details of this exam will be posted in the aula virtual of the subject.
20 %
Presentation and defence of individual or group assignments
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
45 %
Author: Altman, A.
Title: Agricultural biotechnology
Editorial: Marcel Dekker
Publication Date: 1998
ISBN: 0824794397
Author: Gupta, S. Dutta, Ibaraki, Yasuomi
Title: Plant tissue culture engineering
Editorial: Springer
Publication Date: 2006
ISBN: 9781402035944
Author: Davey, Michael R.
Title: Plant cell culture essential methods
Editorial: Wiley-Blackwell,
Publication Date: 2010
ISBN: 9780470686485
Aula virtual (several research articles related with the different topics of this subject will be made available on the aula virtual)
http://plant-tc.cfans.umn.edu/listserv/