Name: ADVANCED IN VITRO CULTURE TECHNIQUES: MICROPROPAGATION AND PRODUCTION OF COMPOUNDS
Code: 229202006
Type: Elective
ECTS: 4
Length of subject: Per term
Semester and course: 2nd Year - First term
Speciality:
Language: English
Mode of study: On-site class
Lecturer data: FERRER AYALA, MARÍA ÁNGELES
Knowledge area: Fisiología Vegetal
Department: Ingeniería Agronómica
Telephone: 968325535
Email: mangeles.ferrer@upct.es
Office hours and location:
lunes - 16:00 / 17:00
EDIFICIO DE ETSI AGRONÓMICA, planta 2, Despacho 2.19
Se recomienda concertar la tutoría por correo electrónico, tanto dentro, como fuera de la fecha y horario indicados. Las tutorías también se podrán realizar mediante la plataforma Teams
Qualifications/Degrees:
Academic rank in UPCT: Catedrática de Universidad
Number of five-year periods: 6
Number of six-year periods: 5 de investigación y 1 de transferencia
Curriculum Vitae: Full Profile
Responsible for the groups: G2
1. Interpretar, discutir críticamente y transmitir oralmente artículos de investigación sobre el
tema.
2. Detectar errores de diseño y buscar soluciones a los mismos en instalaciones dedicadas al
cultivo in vitro de material vegetal.
3. Dominar las técnicas de manipulación empleadas en el cultivo in vitro de material vegetal
con el fin de multiplicarlo, conservarlo y obtener metabolitos de interés industrial.
1. Conocer los factores que determinan las respuestas del material vegetal cultivado in vitro y
la forma de modificarlos.
2. Analizar, explicar y discutir las características de instalaciones y procesos dedicados al cultivo in vitro.
3. Proponer procesos nuevos, o mejoras de los existentes, para optimizar y rentabilizar la
producción de plantas y/o la producción de metabolitos por material cultivado in vitro
1. Instalaciones y equipamiento de un laboratorio de micropropagación. 2. Micropropagación. Automatización y cultivo a gran escala. 3. Limitaciones de la micropropagación vegetal. 4. Problemas durante el establecimiento y cultivo: Contaminación, reacciones hipersensibles, hiperhidricidad, heterotrofía. 5. Problemas tras la transferencia a condiciones ex vitro. 6. Aplicaciones de las técnicas de micropropagación. 7. Impacto económico de la micropropagación. Principales productores y mercado de plantas micropropagadas. 8. Introducción al estudio del potencial biosintético de las plantas. Aprovechamiento industrial. 9. Técnicas especiales de producción de metabolitos: Adición de precursores, biotransformación, elicitación, cultivos mixtos. 10. Cultivos a escala industrial: Tipos de biorreactores, operación con biorreactores. 11. Aplicaciones: Obtención de colorantes, antioxidantes y otros productos de interés farmacéutico. Obtención de biopolímeros. Obtención de proteínas. Anticuerpos.
Unit 1: Introduction and requeriments
C1. Introduction
C2. Facilities and equipment in a micropropagation laboratory
C3. Aseptic work conditions
Unit 2: Micropropagation: methods, limitacions and aplications
C4. Micropropagation steps
C5. Multiplication methods
C6. Automation and large-scale cultures
C7. Plant micropropagation limitations
C8. Common problems associated with micropropagation
C9. Applications
Unit 3: Biosynthetic potential of plants
C10. Introduction to the study of the plant¿s biosynthetic potential
C11. In vitro culture of plant cells, tissues and organs
C12. Special productions techniques: addition of precursors, elicitation, biotransformation, mixed cultures
C13. Industrial scale cultures
C14. Applications
Unit 1-3
Practice 1 (P1). Construction of a bioreactor for the micropropagation of plant material and monitoring of crops (4 h). Practice 2 (P2). Elicitation of cell cultures and analysis of the production of bioactive compounds (4 h). Practice 3 (P3). Establishment of root crops for the production of secondary metabolites (2 h).
Promoting the continuous improvement of working and study conditions of the entire university community is one the basic principles and goals of the Universidad Politécnica de Cartagena. Such commitment to prevention and the responsibilities arising from it concern all realms of the university: governing bodies, management team, teaching and research staff, administrative and service staff and students. The UPCT Service of Occupational Hazards (Servicio de Prevención de Riesgos Laborales de la UPCT) has published a "Risk Prevention Manual for new students" (Manual de acogida al estudiante en materia de prevención de riesgos), which may be downloaded from the e-learning platform ("Aula Virtual"), with instructions and recommendations on how to act properly, from the point of view of prevention (safety, ergonomics, etc.), when developing any type of activity at the University. You will also find recommendations on how to proceed in an emergency or if an incident occurs. Particularly when carrying out training practices in laboratories, workshops or field work, you must follow all your teacher's instructions, because he/she is the person responsible for your safety and health during practice performance. Feel free to ask any questions you may have and do not put your safety or that of your classmates at risk.
Class in conventional classroom: theory, problems, case studies, seminars, etc
Presentation of the lecture. Resolve queries from students. To develop theoretical knowledge based on concepts and theories
Problem solving class: Activities consisting of training sessions to develop practical or applied knowledge based on problem solving
exercises, or practical cases
24
100
Class in laboratory: practical classes / internships
Attendance to lab practice. Use lab equipment properly. Collet lab data. Acquire high-level science skills.
10
100
Class in the field or open classroom (technical visits, lectures, etc.).
Visit a company or research centre engaged in plant cell in vitro culture
2
100
Assessment activities (continuous assessment system)
Continuous assessment system
4
100
Assessment activities (final assessment system)
Attendance to the exams
2
100
Tutorials
Making suggestions for improvement of student work. Resolve doubts.
9
50
Student work: study or individual or group work
Preparation oral exposition
Preparation a formal lab report.
Preparation oral exposition.
Literature search
Self-study: Study the lectures
69
0
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions
25 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
15 %
Presentation and defence of individual or group assignments
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
60 %
Individual test (oral or written)
Individual test (oral or written)
Multiple choice questions and short questions
25 %
Evaluation of practical sessions, visits and seminars based on reports and corresponding documents
Lab report assessment will be done taking into account the criteria established previously in the
rubric.
If students want to improve their lab marks, they can do a practice exam. This exam will covered the basic aspects addressed in the lab sessions.
All the material and details of this exam will be posted in the aula virtual of the subject.
15 %
Presentation and defence of individual or group assignments
Oral exposition assessment will be done taking into account the criteria established previously in the rubric
60 %
Author: Altman, A.
Title: Agricultural biotechnology
Editorial: Marcel Dekker
Publication Date: 1998
ISBN: 0824794397
Author: Davey, Michael R.
Title: Plant cell culture essential methods
Editorial: Wiley-Blackwell,
Publication Date: 2010
ISBN: 9780470686485
Author:
Title: Plant tissue culture engineering
Editorial: Springer
Publication Date: 2006
ISBN: 9781402035944
Aula virtual (several research articles related with the different topics of this subject will be made available on the aula virtual)
http://plant-tc.cfans.umn.edu/listserv/